Work

Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR

Year: 2001

Type: article

Abstract: To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from Pediococcus acidilactici for one mismatch and Micrococcus luteus for the perfect match. PCRs showed that the bias was reduced at lower temperatures. An environmental sample was also examined.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.67.8.3753-3755.2001

Authors: Kousuke Ishii, Manabu Fukui

Institution: Tokyo Metropolitan University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Ministry of Education, Culture, Sports, Science and Technology

Award: G4957308217