Directed differentiation of pluripotent cells to neural lineages: homogeneous formation and differentiation of a neurectoderm population
Year: 2002
Type: article
Abstract: During embryogenesis the central and peripheral nervous systems arise from a neural precursor population, neurectoderm, formed during gastrulation. We demonstrate the differentiation of mouse embryonic stem cells to neurectoderm in culture, in a manner which recapitulates embryogenesis, with the sequential and homogeneous formation of primitive ectoderm, neural plate and neural tube. Formation of neurectoderm occurs in the absence of extraembryonic endoderm or mesoderm and results in a stratified epithelium of cells with morphology, gene expression and differentiation potential consistent with positionally unspecified neural tube. Differentiation of this population to homogeneous populations of neural crest or glia was also achieved. Neurectoderm formation in culture allows elucidation of signals involved in neural specification and generation of implantable cell populations for therapeutic use.
Source: Development
Authors: Joy Rathjen, Bryan P. Haines, Kathryn M. Hudson, Antonietta Nesci, Stephanie Dunn, Peter D. Rathjen
Institutions: Institute of Cancer Research, The University of Adelaide
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2142769704