Multiplex PCRs for Identification of Escherichia coli Virulence Genes
Year: 2000
Type: article
Abstract: PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
Source: Journal of Clinical Microbiology
Authors: M. A. Pass, R. Odedra, R. M. Batt
Institutions: The University of Queensland, Royal Veterinary College
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2142872567