Work

SRA5 Encodes the Low- K m Cyclic AMP Phosphodiesterase of Saccharomyces cerevisiae

Year: 1988

Type: article

Abstract: sra5 mutations in Saccharomyces cerevisiae were previously shown to suppress the inefficient growth of ras2 strains on nonfermentable carbon sources and to result in deficient low-Km cyclic AMP (cAMP) phosphodiesterase activity. We have cloned SRA5 by complementation. It maps to the right arm of chromosome XV, tightly linked to PRT1, and its sequence matches the sequence of PDE2, encoding the low-Km cAMP phosphodiesterase. Disruptions of SRA5 allowed ras1 ras2 strains to grow either on rich media supplemented with cAMP or on minimal media without exogenous cAMP. sra5 strains failed to survive prolonged nitrogen starvation in the presence of exogenous cAMP.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.8.1.505

Authors: R B Wilson, K Tatchell

Institution: University of Pennsylvania

Language: English

Topic: Phosphodiesterase function and regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of General Medical Sciences

Awards: G1847263141, G437146526, Medical Scientist Training Program, GENETIC ANALYSIS OF PROTEIN PHOSPHATASE 1 IN YEAST