Work

Extending the loop design for two-channel microarray experiments

Year: 2006

Type: article

Abstract: The loop design of Kerr and Churchill is a clever application of incomplete blocks of size 2 to two-channel microarray experiments. In this paper, we extend the loop design to include more replicates, biological and technical replication, multi-factor experiments, and blocking. Loop and extended loop designs are shown to be more efficient than the reference design for any given number of arrays. We also show that adding new treatments to a loop design requires the same number of additional arrays as adding treatments to a reference design, with a greater gain in power. Given the flexibility of extended loop designs and their power, we propose that these should be the designs of choice for most experiments using two-channel microarrays.

Source: Genetics Research

DOI: 10.1017/s0016672307008476

Authors: NAOMI S. ALTMAN, JUN HUA

Institution: Pennsylvania State University

Language: English

Topic: Optimal Experimental Design Methods

Subfield: Management Science and Operations Research

Field: Decision Sciences

Domain: Social Sciences

Open Access status: bronze

Funders: National Science Foundation, Division of Biological Infrastructure

Award: The Floral Genome Project