Work

Expression ofβ-galactosidase from a hybrid promoter: operator element inEscherichia coli

Year: 1993
Type: article
Abstract: A hybrid trpPO:lacO regulatory sequence was cloned upstream of a promoterless lacZ gene and recombined onto a lambda bacteriophage. Escherichia coli lysogens representing the four possible phenotypes for lacI and trpR were constructed and the synthesis of beta-galactosidase was assayed under various growth conditions. The results illustrated that both control elements could be efficiently and independently regulated by the addition or omission of appropriate accessory molecules.
Language: English
Subfield: Genetics
Open Access status: bronze
OpenAlex ID: W2143378306
FWCI:
Cites: 12
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Related to: 10