Work

Essential requirement of reduced glutathione (GSH) for the anti-oxidant effect of the flavonoid quercetin

Year: 2005

Type: article

Abstract: We have analyzed the anti- or pro-oxidant effects of the flavonoid quercetin (QU) by evaluating, in U937 cell line, hydrogen peroxide (H(2)O(2)), superoxide anion reduced glutathione (GSH) content, mitochondrial membrane potential, DNA content, phosphatidylserine exposure on the outer face of the plasma membrane and cell viability. Polychromatic flow cytometry was used to evaluate in the same cells several functional parameters. For short periods of treatment QU exerted an anti-oxidant effect (decrease in H(2)O(2) levels), whereas for long periods it showed a pro-oxidant activity (increase in ). In these conditions, GSH content was reduced, and this correlated with a lack of anti-oxidant activity of QU, which in turn could be correlated with proapoptotic activity of this molecule. Thus, QU can exert different effects (anti-/prooxidant) depending on exposure times and oxidative balance, and in particular on stores of GSH.

Source: Free Radical Research

DOI: 10.1080/10715760500306935

Authors: Roberta Ferraresi, Leonarda Troiano, Erika Roat, Enrico Lugli, Elisa Nemes, Milena Nasi, Marcello Pinti, Maria I. Garcia Fernandez, Edwin L. Cooper, Andrea Cossarizza

Institutions: University of Modena and Reggio Emilia, Universidad de Málaga, University of California, Los Angeles

Language: English

Topic: Phytochemicals and Antioxidant Activities

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2143635385

FWCI: 2.738

Cites: 43

Cited by: 98

Related to: 10