Work

DNA Conjugated SWCNTs Enter Endothelial Cells via Rac1 Mediated Macropinocytosis

Year: 2012

Type: article

Abstract: Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.

Source: Nano Letters

DOI: 10.1021/nl204058u

Authors: Santanu Bhattacharya, Daniel Roxbury, Xun Gong, Debabrata Mukhopadhyay, Anand Jagota

Institutions: Mayo Clinic, Lehigh University

Language: English

Topic: Graphene and Nanomaterials Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute