Src homology 2 domain-containing inositol-5’ phosphatase in a murine model of Amyotrophic Lateral Sclerosis
Year: 2009
Type: article
Abstract: As Src homology-2 domain-containing inositol-5' phosphatase (SHIP-1) expression has been implicated in inflammation, immunoreactivity against SHIP-1 was evaluated in mice over-expressing mutant superoxide dismutase (mSOD-1) and wild-type (wt) mice.Spinal cord sections were examined at 11 weeks, 15 weeks of age and at end-stage.SHIP-1 immunoreactivity was detected in mSOD-1 mice at end-stage when considerable motor disability is evident, and at 15 weeks, but not in wt mice.At 15 weeks, SHIP-1 was prominent in the ventral horn, and by end-stage, immunoreactivity was detected throughout the ventral and dorsal regions.SHIP-1 localization was explored further using SHIP-1 immunoreactivity and antibodies directed against microglia and astrocytes.SHIP-1 was not detected in microglia but was detected in astrocytes and this cell specific localization of immunoreactivity suggests that astrocytes can express SHIP-1.The expression of SHIP-1 in astrocytes may be involved in the pathogenesis of murine amyotrophic lateral sclerosis.welcoming into his laboratory and offering me an opportunity to challenge myself through the undertaking of this project, and for this continual support during my research.I would also like to thank Dr. Peter Ruben, for the guidance and he has offered me throughout the course of my graduate work at SFU.Of note, I am extremely grateful for the skills and knowledge I have taken away from my research experience.I owe great thanks to Coral Lewis for her willingness to teach me numerous laboratory procedures, and for the dedicated effort she demonstrated through all levels of the project.I would also like
Source: Summit (Simon Fraser University)
Author: Heather Anne Parrott
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2145266276