Work

Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening

Year: 2007

Type: article

Abstract: A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.01864-07

Authors: Jonathan E. Schmitz, Anu Daniel, Mattias Collin, Raymond Schuch, Vincent A. Fischetti

Institution: Rockefeller University

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases