Role of Protein Kinase G in Growth and Glutamine Metabolism of Mycobacterium bovis BCG
Year: 2005
Type: article
Abstract: The survival of pathogenic mycobacteria in macrophages requires the eukaryotic enzyme-like serine/threonine protein kinase G. This kinase with unknown specificity is secreted into the cytosol of infected macrophages and inhibits phagosome-lysosome fusion. The pknG gene is the terminal gene in a putative operon containing glnH, encoding a protein potentially involved in glutamine uptake. Here, we report that the deletion of pknG did not affect either glutamine uptake or intracellular glutamine concentrations. In vitro growth of Mycobacterium bovis BCG lacking pknG was identical to that of the wild type. We conclude that in M. bovis BCG, glutamine metabolism is not regulated by protein kinase G.
Source: Journal of Bacteriology
Authors: Liem Nguyen, Anne Walburger, Edith Houben, Anil Koul, Stefan Muller, Monika Morbitzer, Bert Klebl, Giorgio Ferrari, Jean Pieters
Institution: University of Basel
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2145754107