Early Inhibition of Glycosylation with Deoxynojirimycin Has No Effect on the Replication of Flavivirus West Nile
Year: 1994
Type: article
Abstract: : Deoxynojirimycin (dNM) is a specific and reversible inhibitor of the trimming ac-tions of glucosidase I and II in the rough endoplasmic reticulum. The importance of the ef-fects of dNM on the replication of West Nile (sarafend- glycosylated) and West Nile (Wengler- nonglycosylated) viruses were studied. It was found that dNM treatment in-crease the molecular weights of the E and prM proteins in both virus strains without affec-ting the yields of the virus. The intracellular transport of the E protein from the rough en-doplasmic reticulum to Golgi apparatus was not affected despite the change in molecular weights. It would appear that the prevention of removal of glucose molecules by dNM did not affected the maturation of E protein or the virus. The envelope (E) protein molecules of flavivirus have a role in the attachment and en-try of the virus (Collins and Porter field, 1985). Despite the role of the E protein, evidence have shown that some mature viruses have glycosylated envelope (E) proteins but are absent in other viruses (Wengler et al, 1985; Wright, 1982). Little is known about the significance of glycosylation in the process of maturation of viruses. Over the past decade, there has been wide-spread use of inhibitors of N-linked oligosaccharide processing to study their effects
Authors: Mah Lee Ng, Lay Tin Aw
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2145842667