Six3 repression of Wnt signaling in the anterior neuroectoderm is essential for vertebrate forebrain development
Year: 2003
Type: preprint
Abstract: In vertebrate embryos, formation of anterior neural structures requires suppression of Wnt signals emanating from the paraxial mesoderm and midbrain territory. In Six3(-/-) mice, the prosencephalon was severely truncated, and the expression of Wnt1 was rostrally expanded, a finding that indicates that the mutant head was posteriorized. Ectopic expression of Six3 in chick and fish embryos, together with the use of in vivo and in vitro DNA-binding assays, allowed us to determine that Six3 is a direct negative regulator of Wnt1 expression. These results, together with those of phenotypic rescue of headless/tcf3 zebrafish mutants by mouse Six3, demonstrate that regionalization of the vertebrate forebrain involves repression of Wnt1 expression by Six3 within the anterior neuroectoderm. Furthermore, these results support the hypothesis that a Wnt signal gradient specifies posterior fates in the anterior neural plate.
Source: Genes & Development
DOI: 10.1101/gad.1059403
Authors: Oleg V. Lagutin, Changqi C. Zhu, Daisuke Kobayashi, Jacek Topczewski, Kenji Shimamura, Luis Puelles, Helen R.C. Russell, Peter J. McKinnon, Lilianna Solnica-Krezel, Guillermo Oliver
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funders: American Lebanese Syrian Associated Charities, Ministry of Education, Culture, Sports, Science and Technology, National Institutes of Health, National Eye Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Neurological Disorders and Stroke
OpenAlex ID: W2146111983