Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expression
Year: 2011
Type: article
Abstract: We describe a new method that allows cloning of double-stranded RNAs (dsRNAs) that are generated in RNase protection experiments. We demonstrate that the mouse C/D box snoRNA MBII-85 (SNORD116) is processed into at least five shorter RNAs using processing sites near known functional elements of C/D box snoRNAs. Surprisingly, the majority of cloned RNAs from RNase protection experiments were derived from endogenous cellular RNA, indicating widespread antisense expression. The cloned dsRNAs could be mapped to genome areas that show RNA expression on both DNA strands and partially overlapped with experimentally determined argonaute-binding sites. The data suggest a conserved processing pattern for some C/D box snoRNAs and abundant expression of longer, non-coding RNAs in the cell that can potentially form dsRNAs.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkr684
Institutions: University of Kentucky, Institució Catalana de Recerca i Estudis Avançats, Universitat Pompeu Fabra, Cold Spring Harbor Laboratory, Ingénierie Moléculaire, Cellulaire et Physiopathologie, Université Henri Poincaré, Université de Lorraine, The University of Texas at Dallas, Tsinghua University
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,992
OpenAlex ID: W2146153978