Work

Removal of AMPA Receptors (AMPARs) from Synapses Is Preceded by Transient Endocytosis of Extrasynaptic AMPARs

Year: 2004

Type: article

Abstract: AMPA receptors (AMPARs) are dynamically regulated at synapses, but the time course and location of their exocytosis and endocytosis are not known. Therefore, we have used ecliptic pHluorin-tagged glutamate receptor 2 to visualize changes in AMPAR surface expression in real time. We show that synaptic and extrasynaptic AMPARs respond very differently to NMDA receptor activation; there is a rapid internalization of extrasynaptic AMPARs that precedes the delayed removal of synaptic AMPARs.

Source: Journal of Neuroscience

DOI: 10.1523/jneurosci.1042-04.2004

Authors: Michael C. Ashby, Sarah A. De La Rue, G. Scott Ralph, James Uney, Graham L. Collingridge, Jeremy M. Henley

Institutions: University of Bristol, Medical Research Council, Oxford BioMedica (United Kingdom)

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Funders: Wellcome Trust, European Commission, Medical Research Council

Awards: G4572004107, Investigating the function of protein chaperones in neurones using advanced viral transfection technologies.