Work

Mutational Analysis of the vacA Promoter Provides Insight into Gene Transcription in Helicobacter pylori

Year: 1999

Type: article

Abstract: Analysis of 12 Helicobacter pylori promoters indicates the existence of a consensus -10 hexamer (TAtaaT) but little conservation of -35 sequences. In this study, mutations in either the H. pylori vacA -10 region or the -35 region resulted in decreased vacA transcription and suggested that an extended -10 motif is utilized. Thus, despite the lack of a -35 consensus sequence for H. pylori promoters, the -35 region plays a functional role in vacA transcription.

Source: Journal of Bacteriology

DOI: 10.1128/jb.181.7.2261-2266.1999

Authors: Mark H. Forsyth, Timothy L. Cover

Institutions: Vanderbilt University, Nashville VA Medical Center

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: Structure and function of Helicobacter pylori VacA, G396062782, G4513176822, Cancer Center Support Grant