Work

Co‐isolation of extracellular vesicles and high‐density lipoproteins using density gradient ultracentrifugation

Year: 2014

Type: article

Abstract: Extracellular vesicles (EVs) facilitate intercellular communication by carrying bioactive molecules such as proteins, messenger RNA, and micro (mi)RNAs. Recently, high-density lipoproteins (HDL) isolated from human plasma were also reported to transport miRNA to other cells. HDL, when isolated from human plasma, ranges in density between 1.063 and 1.21 g/mL, which grossly overlap with the reported density of EVs. Consequently, HDL and EV will be co-isolated when using density gradient ultracentrifugation. Thus, more stringent isolation/separation procedures of EV and HDL are essential to know their relative contribution to the pool of circulating bioactive molecules.

Source: Journal of Extracellular Vesicles

DOI: 10.3402/jev.v3.23262

Authors: Yuana Yuana, Johannes Levels, Anita Grootemaat, Auguste Sturk, Rienk Nieuwland

Institutions: Amsterdam UMC Location University of Amsterdam, Amsterdam University Medical Centers, University of Amsterdam

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $4,410