Work

Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery

Year: 2008

Type: article

Abstract: The ability to inject exogenous material as well as to alter subcellular structures in a minimally invasive manner using a laser microbeam has been useful for cell biologists to study the structure-function relationship in complex biological systems. We describe a quantitative phase laser microsurgery system, which takes advantage of the combination of laser microirradiation and short-coherence interference microscopy. Using this method, quantitative phase images and the dynamic changes of phase during the process of laser microsurgery of red blood cells (RBCs) can be evaluated in real time. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intracellular structures, being exposed to the laser microbeam. Such quantitation was not possible using conventional phase-contrast microscopy.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.2997375

Authors: Lingfeng Yu, Samarendra K. Mohanty, Gangjun Liu, Suzanne Genc, Zhongping Chen, Michael W. Berns

Institutions: University of California, Irvine, Raja Ramanna Centre for Advanced Technology, Beckman Laser Institute and Medical Clinic

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: bronze