Work

Identification of Two NF-κB Sites in Mouse CD95 Ligand (Fas Ligand) Promoter: Functional Analysis in T Cell Hybridoma

Year: 1998

Type: article

Abstract: Fas ligand (FasL) gene expression is critically involved in peripheral T cell tolerance and lymphocyte homeostasis. Previous studies have suggested that nuclear translocation of NF-kappaB during T cell activation is a critical event for FasL gene activation. In the present study we have identified two NF-kappaB sites (designated FasL-kappaB1 and FasL-kappaB2) on the promoter (approximately 700 bp) of FasL. The NF-kappaB sites were identified by electrophoretic mobility shift assay. Transient transfection reporter analyses showed that the FasL promoter activity was comparable between a construct that contains both sites and a shorter construct (433 bp) that contains only the FasL-kappaB1 site. Furthermore, elimination of FasL-kappaB1 by site-directed mutagenesis significantly inhibited FasL promoter activity. These observations provide strong evidence that NF-kappaB directly binds to the FasL-kappaB1 site and up-regulates FasL gene expression.

Source: The Journal of Immunology

DOI: 10.4049/jimmunol.161.7.3469

Authors: Ken Matsui, Alan Fine, Bangmin Zhu, Ann Marshak-Rothstein, Shyr-Te Ju

Institution: Boston University

Language: English

Topic: NF-κB Signaling Pathways

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: National Institute of Allergy and Infectious Diseases