Work

Construction of Mobilizable Mini-Tn 7 Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy Promoter lux Reporter Analysis

Year: 2013

Type: article

Abstract: We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.00640-13

Authors: F. Heath Damron, Elizabeth S. McKenney, Mariette Barbier, George W. Liechti, Herbert P. Schweizer, Joanna B. Goldberg