Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates
Year: 2012
Type: article
Abstract: Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Awards: Platform Grant Support for Materials Characterisation at Oxford, Imaging Membrane Potential via Second Harmonic Generation, Fast remote focussing for three-dimensional microscopy, Ion Channels and Diseases of Electrically Excitable Cells., Fast remote focusing for three-dimensional imaging of biological tissues., Oxford Centre for Neural Circuits and Behaviour., Adaptive optics for three-dimensional microscopy and photonic engineering
OpenAlex ID: W2147549961