Work

The 1-Carbamoyl-2-oxo-4,5-dihydroxyimidazolidine Component of ROS-Induced DNA Damage in White Blood Cells

Year: 2010

Type: article

Abstract: The 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification of cytosine is a known base modification produced in vitro by oxidative stress. However, the presence of this modification in vivo has not been established. In this study the introduction of this base modification into dinucleoside monophosphates was accomplished using the Fenton reaction. Subsequently, the modification was produced in short isotopically labeled oligomers. Labeled tetramers bearing the lesion were used as internal standards for LC-MS/MS determinations of the base modification in the DNA of white blood cells from healthy donors. The background level of the 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification of cytosine was found to be larger than the levels of the formamide and thymine glycol base modifications.

Source: Radiation Research

DOI: 10.1667/rr2087.1

Authors: Herbert Iijima, Helen B. Patrzyc, Edwin E. Budzinski, Jean B. Dawidzik, Harold G. Freund, Harold C. Box

Institution: Roswell Park Comprehensive Cancer Center

Language: English

Topic: Carcinogens and Genotoxicity Assessment

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Cancer Institute