Work

Use of BODIPY (493/503) to Visualize Intramuscular Lipid Dropletsin Skeletal Muscle

Year: 2011

Type: article

Abstract: Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization. We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle.

Source: BioMed Research International

DOI: 10.1155/2011/598358

Authors: Espen E. Spangenburg, Stephen J. P. Pratt, Lindsay M. Wohlers, Richard M. Lovering

Institutions: University of Maryland, College Park, University of Maryland, Baltimore

Language: English

Topic: Lipid metabolism and biosynthesis

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,680

Funders: National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases