Work

A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation

Year: 2015

Type: article

Abstract: Driving human pluripotent stem cells (hPSCs) into specific lineages is an inefficient and challenging process. We show that a potent Src inhibitor, PP1, regulates expression of genes involved in the G1 to S phase transition of the cell cycle, activates proteins in the retinoblastoma family, and subsequently increases the differentiation propensities of hPSCs into all three germ layers. We further demonstrate that genetic suppression of Src regulates the activity of the retinoblastoma protein and enhances the differentiation potential of hPSCs across all germ layers. These positive effects extend beyond the initial germ layer specification and enable efficient differentiation at subsequent stages of differentiation.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.201502035

Authors: Sundari Chetty, Elise N. Engquist, Elie Mehanna, Kathy O. Lui, Alexander M. Tsankov, Douglas A. Melton

Institutions: Harvard University, Harvard Stem Cell Institute, Broad Institute, Howard Hughes Medical Institute

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Diabetes and Digestive and Kidney Diseases