Work

LC‐MS Data Processing with MAVEN: A Metabolomic Analysis and Visualization Engine

Year: 2012

Type: article

Abstract: MAVEN is an open-source software program for interactive processing of LC-MS-based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple reaction monitoring data or high-resolution full-scan mass spectrometry data. It automatically detects and reports peak intensities for isotope-labeled metabolites. Menu-driven, click-based navigation allows visualization of raw and analyzed data. Here we provide a User Guide for MAVEN. Step-by-step instructions are provided for data import, peak alignment across samples, identification of metabolites that differ strongly between biological conditions, quantitation and visualization of isotope-labeling patterns, and export of tables of metabolite-specific peak intensities. Together, these instructions describe a workflow that allows efficient processing of raw LC-MS data into a form ready for biological analysis.

Source: Current Protocols in Bioinformatics

DOI: 10.1002/0471250953.bi1411s37

Authors: Michelle F. Clasquin, Eugene Melamud, Joshua D. Rabinowitz

Institutions: Merck & Co., Inc., Rahway, NJ, USA (United States), Pfizer (United States)

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Award: Role of Tumor and Stromal Cell Metabolism in Stress Adaptation and Progression