Work

Cloning an iron-regulated metal transporter from rice

Year: 2002

Type: article

Abstract: Rice cDNA and genomic libraries were screened in order to clone an Fe(II) transporter gene. A cDNA clone highly homologous to the Arabidopsis Fe(II) transporter gene IRT1 was isolated from Fe-deficient rice roots. The cDNA clone was named OsIRT1. A genomic clone corresponding to the cDNA was also obtained, sequenced and analysed. When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron-uptake mutant. Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe- and Cu-deficiency. This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions.

Source: Journal of Experimental Botany

DOI: 10.1093/jxb/erf004

Authors: N. Bughio, Hirotaka Yamaguchi, Naoko K Nishizawa, Hiromi Nakanishi, Satoshi Mori

Institution: The University of Tokyo

Language: English

Topic: Plant Micronutrient Interactions and Effects

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funder: Japan Science and Technology Corporation