Work

Preparative Isolation and Purification of Five Flavonoids From Pogostemon Cablin Benth by High-speed Countercurrent Chromatography and Preparative High-performance Liquid Chromatography

Year: 2011

Type: article

Abstract: High-speed countercurrent chromatography (HSCCC) and preparative high-performance liquid chromatography (prep-HPLC) were successively used for the separation of pogostone and four flavonoids from Pogostemon cablin (Blanco) Benth. An efficient HSCCC separation was achieved on a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (11:5:11:5, v/v/v/v). Three well-separated peaks were obtained in the HSCCC chromatogram. The first and the second fractions each contained two flavonoids which were further separated by preparative HPLC. Consequently, the separation yielded 11.5 mg of 4', 5-Dihydroxy-3', 7-dimethoxyflavanone at a purity of 99%, 20.3 mg of 5- Hydroxy-7, 3', 4'-trimethoxyflavanone at a purity of 98%, 18 mg of 5, 4'-Dihydroxy-3, 7, 3'-trimethoxyflavone at a purity of 96%, and 8 mg of 5-Hydroxy-3, 7, 4'-tetramethoxyflvone at a purity of 98%. The third HSCCC fraction yielded 18.5 mg of pogostone at a purity of 95%. The chemical structures of these compounds were identified by ESI-MS(n), (1)H-NMR, and (13)C-NMR.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826076.2011.580486

Authors: Kang Li, Hongwu Zhang, Huichun Xie, Yong Liang, Xiaohong Wang, Yoichiro Ito

Institutions: Guangdong Pharmaceutical University, South China Normal University, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Natural Science Foundation of China, National Institutes of Health

Award: G4705346009