Characterization and applications of two protease enzymes obtained by culture dependent and independent approaches from mangrove sediments
Year: 2014
Type: dissertation
Abstract: Soil community genomics or metagenomics is employed in this study to analyze the evolutionary related\n-\nness of mangrove microbial community. The metagenomic DNA was isolated from mangrove sediment and \n16SrDNA was amplified using universal primers. The amplicons were ligated into pTZ57R/T cloning vector and transformed \nonto E. coli JM109 host cells. The recombinant plasmids were isolated from positive clones and the insert was confirmed \nby its reamplification. The amplicons were subjected to Amplified Ribosomal DNA Restriction Analysis (ARDRA) using three \ndifferent tetra cutter restriction enzymes namely Sau3A1, Hha1 and HpaII. The 16SrDNA insert were sequenced and their \nidentity was determined. The sequences were submitted to NCBI database and accession numbers obtained. The phylo\n-\ngenetic tree was constructed based on Neighbor-Joining technique. Clones belonged to two major phyla of the bacterial \ndomain, namely Firmicutes and Proteobacteria, with members of Firmicutes predominating. The microbial diversity of the \nmangrove sediment was explored in this manner.
Authors: Helvin Vincent, Bhatt Saritha G
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2151297982