Work

Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example

Year: 2008

Type: article

Abstract: We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.02403-07

Authors: D. Bru, F. Martin-Laurent, L. Philippot

Institutions: Université de Bourgogne, Centre de Microbiologie du Sol et de l'Environnement

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2151308724

FWCI: 4.222

Cites: 16

Cited by: 305

Related to: 10