Year: 2014
Type: article
Abstract: RATIONALE: Cardiac progenitor cells (CPCs) are thought to differentiate into the major cell types of the heart: cardiomyocytes, smooth muscle cells, and endothelial cells. We have recently identified ABI family, member 3 (NESH) binding protein (Abi3bp) as a protein important for mesenchymal stem cell biology. Because CPCs share several characteristics with mesenchymal stem cells, we hypothesized that Abi3bp would similarly affect CPC differentiation and proliferation. OBJECTIVE: To determine whether Abi3bp regulates CPC proliferation and differentiation. METHODS AND RESULTS: In vivo, genetic ablation of the Abi3bp gene inhibited CPC differentiation, whereas CPC number and proliferative capacity were increased. This correlated with adverse recovery after myocardial infarction. In vitro, CPCs, either isolated from Abi3bp knockout mice or expressing an Abi3bp shRNA construct, displayed a higher proliferative capacity and, under differentiating conditions, reduced expression of both early and late cardiomyocyte markers. Abi3bp controlled CPC differentiation via integrin-β1, protein kinase C-ζ, and v-akt murine thymoma viral oncogene homolog. CONCLUSIONS: We have identified Abi3bp as a protein important for CPC differentiation and proliferation.
Source: Circulation Research
Authors: Conrad P. Hodgkinson, Jose A. Gomez, Alan J. Payne, Lunan Zhang, Xiaowen Wang, Sophie Dal-Pra, Richard E. Pratt, Victor J. Dzau
Institutions: Duke University, Duke Medical Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G108157200, G1651367797, G2811218247, Gene Therapy for Long-Term Myocardial Protection, G3591235599, Renin Gene Expression in Cardiovascular Regulation: Mechanisms of JG Hyperplasia, G6483445886, Genetically Altered Mesenchymal Stem Cell; Paracrine Effect on Neovascularization, Sfrp2 and Cardiac Progenitor Cells in Regenerative Response to Ischemic Injury
OpenAlex ID: W2151930288