Work

Localization of phospholamban in smooth muscle using immunogold electron microscopy.

Year: 1988

Type: article

Abstract: Phospholamban, the putative regulator of the Ca2+-ATPase in cardiac sarcoplasmic reticulum, was immunolocalized in canine visceral and vascular smooth muscle. Gently disrupted tissues were labeled with an affinity-purified phospholamban polyclonal antibody and indirect immunogold, using preembedding techniques. The sarcoplasmic reticulum of smooth muscle cells was specifically labeled with patches of immunogold distributed in a nonuniform fashion, while the sarcolemma did not appear to contain any phospholamban. The outer nuclear envelopes were also observed to be heavily labeled with the affinity-purified phospholamban polyclonal antibody. These findings suggest that phospholamban may play a role in the regulation of cytoplasmic and intranuclear calcium levels in smooth muscle cells.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.107.2.555

Authors: D G Ferguson, E F Young, L Raeymaekers, E G Kranias

Institution: University of Cincinnati

Language: English

Topic: Ion channel regulation and function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Heart, Lung, and Blood Institute

Awards: G1276932499, G4574220777, G953412423