Work

A Novel Assay for Quantifying the Number of Plasmids Encapsulated by Polymer Nanoparticles

Year: 2011

Type: article

Abstract: Polymeric nanoparticles are promising for gene therapy and stem cell reprogramming using nonviral vectors. A novel assay utilizing nanoparticle tracking analysis is developed to easily quantify the number of plasmids within polymeric nanoparticles while in aqueous solution. Particles effective at co-transfecting primary human fibroblasts are approximately 100 nm in diameter and contain around 100 plasmids per particle.

Source: Small

DOI: 10.1002/smll.201101718

Authors: Nupura S. Bhise, Ron B. Shmueli, Jose Gonzalez, Jordan J. Green

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Maryland Stem Cell Research Fund, National Cancer Institute