Work

Single-cell migration as studied by scanning electrochemical microscopy

Year: 2015

Type: article

Abstract: Scanning electrochemical microscopy (SECM) was used to study the migration of single live head and neck cancer cells (SCC25). The newly developed graphite paste ultramicroelectrode (UME) showed significantly less fouling in comparison to a 10 μm Pt-UME and thus could be used to monitor and track the migration pattern of a single cell. We also used SECM probe scan curves to measure the morphology (height and diameter) of a single live cancer cell during cellular migration and determined these dimensions to be 11 ± 4 μm and 40 ± 10 μm, respectively. The migration study revealed that cells within the same cell line had a heterogeneous migration pattern (migration and stationary) with an estimated migration speed of 8 ± 3 μm/h. However, serum-starved synchronized cells of the same line were found to have a non-heterogeneous cellular migration pattern with a speed of 9 ± 3 μm/h. Thus, this non-invasive SECM-based technique could potentially be expanded to other cell lines to study cellular biomechanics for improved understanding of the structure-function relationship at the level of a single cell.

Source: Analytical Methods

DOI: 10.1039/c5ay01944c

Authors: J. Ganesh Ummadi, Vrushali S. Joshi, Priya R. Gupta, Arup K. Indra, Dipankar Koley

Institutions: Oregon State University, Oregon Health & Science University, OHSU Knight Cancer Institute

Language: English

Topic: Electrochemical Analysis and Applications

Subfield: Electrochemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Oregon State University, National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases