Work

Microarray probe expression measures, data normalization and statistical validation

Year: 2003

Type: article

Abstract: DNA microarray technology is a high-throughput method for gaining information on gene function. Microarray technology is based on deposition/synthesis, in an ordered manner, on a solid surface, of thousands of EST sequences/genes/oligonucleotides. Due to the high number of generated datapoints, computational tools are essential in microarray data analysis and mining to grasp knowledge from experimental results. In this review, we will focus on some of the methodologies actually available to define gene expression intensity measures, microarray data normalization, and statistical validation of differential expression.

Source: Comparative and Functional Genomics

DOI: 10.1002/cfg.312

Authors: Silvia Saviozzi, Raffaele A. Calogero

Institutions: Ospedale San Luigi Gonzaga, University of Turin

Language: English

Topic: Gene expression and cancer classification

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funder: Ministero dell’Istruzione, dell’Università e della Ricerca

Award: Interpretazione del ruolo fisiologico di p63, uno dei membri della famiglia di p53, attraverso lo studio dei meccanismi di regolazione, la caratterizzazione dell'attività trascrizionale e l'identificazione dei geni bersaglio