Work

Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis

Year: 2000

Type: preprint

Abstract: Bub3 is a conserved component of the mitotic spindle assembly complex. The protein is essential for early development in Bub3 gene-disrupted mice, evident from their failure to survive beyond day 6.5-7.5 postcoitus (pc). Bub3 null embryos appear normal up to day 3.5 pc but accumulate mitotic errors from days 4.5-6.5 pc in the form of micronuclei, chromatin bridging, lagging chromosomes, and irregular nuclear morphology. Null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. The results confirm Bub3 as a component of the essential spindle checkpoint pathway that operates during early embryogenesis.

Source: Genes & Development

DOI: 10.1101/gad.827500

Authors: Paul Kalitsis, Elizabeth Earle, Kerry J. Fowler, K.H. Andy Choo

Institutions: Royal Children's Hospital, Murdoch Children's Research Institute

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Medical Research Council, National Health and Medical Research Council

OpenAlex ID: W2153198916

Cites: 28

Cited by: 245

Related to: 9