Work

Degradation of Azo Dyes by Trametes villosa Laccase over Long Periods of Oxidative Conditions

Year: 2005

Type: article

Abstract: Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulated after 72 h of incubation were analyzed. Liquid chromatography-mass spectrometry (LC-MS) analysis showed the formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerized products that retain azo group integrity. The amino-phenol reactions were also investigated by 13C-nuclear magnetic resonance and LC-MS analysis, and the polymerization character of laccase was shown. This study highlights the fact that laccases polymerize the reaction products obtained during long-term batch decolorization processes with azo dyes. These polymerized products provide unacceptable color levels in effluents, limiting the application of laccases as bioremediation agents.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.71.11.6711-6718.2005

Authors: Andrea Zille, Barbara Górnacka, Astrid Rehorek, Artur Cavaco-Paulo

Institutions: University of Minho, TH Köln - University of Applied Sciences

Language: English

Topic: Enzyme-mediated dye degradation

Subfield: Applied Microbiology and Biotechnology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: green

Funder: Fundação para a Ciência e a Tecnologia