Work

In vitro organogenesis from internodal segments of adult sweet orange plants

Year: 2011

Type: article

Abstract: The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.

Source: Pesquisa Agropecuária Brasileira

DOI: 10.1590/s0100-204x2011000600015

Authors: Meire Menezes Bassan, Francisco de Assis Alves Mourão Filho, Luzia Yuriko Miyata, Beatriz Madalena Januzzi Mendes

Institution: Universidade de São Paulo

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funders: Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundo de Defesa da Citricultura