Work

Genetic recombination of poliovirus in a cell-free system

Year: 1997

Type: article

Abstract: Genetic recombination of plus-strand RNA viruses is an important process for promoting genetic variation. By using genetically marked poliovirus RNAs, we have demonstrated that genetic recombination can occur in a cell-free system that generates infective virus from added poliovirus RNA. Recombinant polioviruses were isolated, and the region of crossing over was roughly mapped. Recombinants could be isolated even under conditions where the yield of viruses from one of the parental RNAs was depressed to levels comparable to or less than the yield of recombinant viruses, an observation suggesting that only one of the recombining RNAs needs to be replication-competent. The generation of poliovirus recombinants in a cell-free system offers new possibilities for studying recombination and evolution of RNA viruses.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.94.25.13786

Authors: Rohit Duggal, Andrea Cuconati, Matthias Gromeier, Eckard Wimmer

Institutions: State University of New York, Brookhaven National Laboratory, Stony Brook University

Language: English

Topic: Evolution and Genetic Dynamics

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: G1185418969, G4354770694, G4677032722, Picornavirus Molecular Biology, G8119709225