Work

Receptor-mediated adherence of Campylobacter pylori to mouse Y-1 adrenal cell monolayers

Year: 1989

Type: article

Abstract: An in vitro adherence assay was developed to study the adherence of Campylobacter pylori to mammalian cells. Strains of C. pylori were isolated from individuals with gastritis. These strains possessed the fibrillar N-acetylneuraminyllactose(neuraminlactose)-binding hemagglutinin (NLBH) and were found to adhere to monolayers of mouse Y-1 adrenal cells. Adherence was rapid, prevented by pretreatment of the Y-1 cells with Clostridium perfringens neuraminidase, and blocked by the neuraminlactose-containing protein fetuin. Electron microscopy by the immunogold technique and with anti-NLBH antibody showed that NLBH was present at the sites of interaction between C. pylori and the Y-1 cells. These results indicate that the Y-1 monolayer is a valid model for receptor-specific adherence of C. pylori.

Source: Infection and Immunity

DOI: 10.1128/iai.57.8.2272-2278.1989

Authors: D G Evans, D J Evans, D Y Graham

Institution: Baylor College of Medicine

Language: English

Topic: Helicobacter pylori-related gastroenterology studies

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Diabetes and Digestive and Kidney Diseases