Work

Double-strand end repair via the RecBC pathway in Escherichia coli primes DNA replication

Year: 1999

Type: preprint

Abstract: To study the relationship between homologous recombination and DNA replication in Escherichia coli, we monitored the behavior of phage lambda chromosomes, repressed or not for lambda gene activities. Recombination in our system is stimulated both by DNA replication and by experimentally introduced double-strand ends, supporting the idea that DNA replication generates occasional double-strand ends. We report that the RecBC recombinational pathway of E. coli uses double-strand ends to prime DNA synthesis, implying a circular relationship between DNA replication and recombination and suggesting that the primary role of recombination is in the repair of disintegrated replication forks arising during vegetative reproduction.

Source: Genes & Development

DOI: 10.1101/gad.13.3.345

Authors: A. Kuzminov, F. W. Stahl

Institution: University of Oregon

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funder: National Science Foundation

Award: Growth, Mutation and Recombination in Bacteriophage