Work

Biases in Illumina transcriptome sequencing caused by random hexamer priming

Year: 2010

Type: article

Abstract: Generation of cDNA using random hexamer priming induces biases in the nucleotide composition at the beginning of transcriptome sequencing reads from the Illumina Genome Analyzer. The bias is independent of organism and laboratory and impacts the uniformity of the reads along the transcriptome. We provide a read count reweighting scheme, based on the nucleotide frequencies of the reads, that mitigates the impact of the bias.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkq224

Authors: Kasper D. Hansen, Steven E. Brenner, Sandrine Dudoit

Institution: University of California, Berkeley

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,992

Funders: National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences