Work

Evolution of CXCR4-Using Human Immunodeficiency Virus Type 1 SF162 Is Associated with Two Unique Envelope Mutations

Year: 2007

Type: article

Abstract: CCR5-using human immunodeficiency virus type 1 (HIV-1) isolates typically gain CXCR4 use via multiple mutations in V3 and often V1/V2 regions of envelope, and patterns of mutations are distinct for each isolate. Here, we report that multiple CXCR4-using variants of a parental CCR5-using HIV-1 isolate, SF162, obtained by either target cell selection or CCR5 inhibition have a common mutation pattern characterized by the same two V3 mutations and that these mutations preexisted in some of the SF162 stocks. These results imply that SF162 has a single pathway for acquiring CXCR4 use and that prolonged culture is sufficient to select for R5X4 variants.

Source: Journal of Virology

DOI: 10.1128/jvi.02310-06

Authors: Yana Kiselyeva, Rebecca Nedellec, Alejandra Ramos, Cristina Pastore, Leonid B. Margolis, Donald E. Mosier

Institutions: National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Scripps Research Institute

Language: English

Topic: HIV Research and Treatment

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Children's National Hospital, National Institutes of Health, National Institute of Allergy and Infectious Diseases