Work

Expression of UGA-Containing Mycoplasma Genes in Bacillus subtilis

Year: 2000

Type: article

Abstract: We used Bacillus subtilis to express UGA-containing Mycoplasma genes encoding the P30 adhesin (one UGA) of Mycoplasma pneumoniae and methionine sulfoxide reductase (two UGAs) of Mycoplasma genitalium. Due to natural UGA suppression, these Mycoplasma genes were expressed as full-length protein products, but at relatively low efficiency, in recombinant wild-type Bacillus. The B. subtilis-expressed Mycoplasma proteins appeared as single bands and not as multiple bands compared to expression in recombinant Escherichia coli. Bacillus mutants carrying mutations in the structural gene (prfB) for release factor 2 markedly enhanced the level of readthrough of UGA-containing Mycoplasma genes.

Source: Journal of Bacteriology

DOI: 10.1128/jb.182.9.2664-2667.2000

Authors: T. R. Kannan, Joel B. Baseman

Institution: The University of Texas at San Antonio Health Science Center

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: G343237486, BIOLOGY AND PATHOGENICITY OF MYCOPLASMA GENITALIUM