Work

Recombination of Plasmids into the Saccharomyces cerevisiae Chromosome Is Reduced by Small Amounts of Sequence Heterogeneity

Year: 1983

Type: article

Abstract: As a model system for studying the properties of mitotic recombination in the yeast Saccharomyces cerevisiae, we have examined recombination between a recombinant plasmid (introduced into the S. cerevisiae cell by transformation) and homologous chromosomal loci. The recombinant plasmids used in these experiments contained S. cerevisiae rRNA genes. We found that the frequency of integrative recombination is sensitive to small amounts of sequence heterogeneity. In addition, the frequency and specificity of these recombination events are affected by the lengths of the interacting homologous DNA sequences.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.3.7.1204

Authors: S Smolik-Utlaut, T D Petes

Institution: University of Chicago

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: Recombination in yeast, Molecular and Cellular Biology Training