Work

Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates

Year: 1995

Type: article

Abstract: We describe a rapid method for subtyping Mycobacterium tuberculosis based on PCR amplification of segments located between two distinct DNA repetitive elements. This method, double-repetitive-element PCR, classified 46 clinical isolates as having 25 distinct patterns; the conventional restriction fragment length polymorphism analysis classified the same isolates as having 23 distinct patterns. The double-repetitive-element PCR is a rapid subtyping method that has a discriminating power similar to that of the restriction fragment length polymorphism method.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.33.5.1383-1384.1995

Authors: C R Friedman, M Y Stoeckle, W D Johnson, L W Riley

Institution: Cornell University

Language: English

Topic: Tuberculosis Research and Epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases