Year: 2014
Type: dissertation
Study design: Observational Study
Abstract: Studies of neutrophilic inflammation in tobacco smokers\nKristina Andelid\nDepartment of Internal medicine and clinical nutrition, Institute of Medicine\nSahlgrenska Academy at University of Gothenburg\nGöteborg, Sweden\nABSTRACT\nThe general aim of this thesis was to characterise markers of neutrophilic inflammation in\nsmokers with and without obstructive pulmonary disease with chronic bronchitis\n(OPD-CB) in a clinically stable state and during exacerbations compared to heathy\ncontrols. Methodology: I) Blood samples were obtained from male smokers without\nairway symptoms and never-smokers at year 0 and 6. II) Non-atopic and atopic,\noccasional-smokers plus never-smokers underwent two bronchoscopies, including\nbronchoalveolar lavage (BAL). III & IV) Smokers with OPD-CB (n=60,) and control\ngroups (n=10 each), underwent blood and sputum sampling every 15: th week and during\nexacerbations for 15 months. Results: I) Blood MPO was higher in smokers than in\nnever- smokers at year 6. MPO was negatively correlated with time after cessation of\nsmoking. II) Gelatinases in BAL fluid were unchanged after acute exposure to tobacco\nsmoke. III) The concentrations of IL-17A and GRO-α protein were lower in blood from\nsmokers with severe OPD-CB and in smokers with OPD-CB colonised with opportunistic\npathogens. IV) In smokers with OPD-CB, blood MPO and NE proteins were increased\nduring exacerbations; the corresponding mRNA was undetectable. Conclusions: Acute\nexposure to tobacco smoke does not exert a pronounced, lasting impact on gelatinases in\nthe airways of occasional-smokers. During stable clinical conditions, neutrophil and MPO\nconcentrations are increased in smokers without OPD-CB and even more so during\nexacerbations in smokers with OPD-CB. In smokers with severe OPD-CB, and in those\ncolonised with opportunistic pathogens, specific neutrophil-associated cytokine signaling\nis down-regulated at the systemic level. The lack of detectable mRNA for MPO and NE in\nthe blood of smokers with OPD-CB makes the location of production uncertain for these\nmarkers of neutrophil activity.\nKeywords: smoking, neutrophils, inflammation\nISBN: 978-91-628-9216-6 http://hdl.handle.net/2077/36915
Author: Kristina Andelid
Institution: University of Gothenburg
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2155737971