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A novel role for lipid droplets in the organismal antibacterial response

Year: 2012
Type: article
Abstract: We previously discovered histones bound to cytosolic lipid droplets (LDs); here we show that this forms a cellular antibacterial defense system. Sequestered on droplets under normal conditions, in the presence of bacterial lipopolysaccharide (LPS) or lipoteichoic acid (LTA), histones are released from the droplets and kill bacteria efficiently in vitro. Droplet-bound histones also function in vivo: when injected into Drosophila embryos lacking droplet-bound histones, bacteria grow rapidly. In contrast, bacteria injected into embryos with droplet-bound histones die. Embryos with droplet-bound histones displayed more than a fourfold survival advantage when challenged with four different bacterial species. Our data suggests that this intracellular antibacterial defense system may function in adult flies, and also potentially in mice.DOI:http://dx.doi.org/10.7554/eLife.00003.001.
Source: eLife
DOI: 10.7554/elife.00003
Authors: Preetha Anand, Silvia Cermelli, Zhihuan Li, Adam Kassan, Marta Bosch, Robilyn Sigua, Lan Huang, Andre J Ouellette, Albert Pol, Michael A Welte, Steven P Gross
Language: English
Subfield: Microbiology
Open Access status: gold
Estimated APC paid: $2,500
Funders: National Science Foundation, Princeton University, Ministerio de Ciencia e Innovación, National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences, National Center for Research Resources
Awards: G1393532939, Lipid droplets as sites for protein sequestration, Confocal Microscope for University of Rochester Biology Dept, In vivo regulation of bi-directional transport, Developmental control of organelle transport, Proteomics of the Proteasome Interacting Networks
OpenAlex ID: W2155750054
FWCI: 6.005
Cites: 44
Cited by: 134
Related to: 10