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Rapid Detection of Mycobacterium tuberculosis Complex and Rifampin Resistance in Smear-Negative Clinical Samples by Use of an Integrated Real-Time PCR Method

Year: 2010

Type: article

Abstract: Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.01831-10

Authors: Raquel Moure, Laura Muñoz, Miriam Torres, Miguel Santin, Rogelio Martín, Fernando Alcaide

Institutions: Bellvitge University Hospital, Institut d'Investigació Biomédica de Bellvitge, Universitat de Barcelona

Language: English

Topic: Tuberculosis Research and Epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2155790306

FWCI: 11.47

Cites: 22

Cited by: 167

Related to: 10