Year: 2002
Type: dissertation
Abstract: 7 REVIEW OF THE LITERATURE 9 1 THE CELL DIVISION CYCLE 9 1.1 Cyclin-dependent kinases (Cdks) 9 1.2 Regulation of Cdks 10 2 CDK-ACTIVATING KINASES 11 2.1 Menage a trois (MAT1) 12 2.2 The Cdk-activating kinase – from mammals to yeast 13 3 CDK ACTIVATION MEETS BASAL TRANSCRIPTION 13 3.1 Transcription factor IIH (TFIIH) 14 3.2 Substrates of the TFIIH kinase 15 4 HISTIDINE TRIAD (HIT) GENE FAMILY 17 4.1 The origin of Hint 18 4.2 FHIT 18 4.3 GalT 19 4.4 Novel members of the HIT family: aprataxin, Hint2, and Hint3 19 4.5 HIT proteins – from structure to function 20 4.6 Other Hint-interacting cellular proteins 22 AIMS OF THE STUDY 23 MATERIALS AND METHODS 24 Generation of genetically engineered Mat1 (I, II) and Hint mice (IV) 24 Generation and analysis of Hint;Fhit mice (IV) 24 Generation of S. cerevisiae HNT1 disruptant (III) 24 PCR genotyping (I, II, III, IV) 25 Protein analysis (I, II, III, IV) 26 Immunofluorescence and immunohistochemistry (I, II, III) 26 BrdU labeling (I, II) 26 Blastocyst outgrowths (I) 27 Microinjection (I) 27 Histology (II, IV) 27 Transmission electron microscopy (II) 27 s-galactosidase and alkaline phosphatase staining (II) 27 Yeast strains and culture conditions (III) 28 Yeast transformation (III) 28 Production of GST proteins and solution binding assay (III) 28 Transfection (III) 29 In vitro kinase assay (III, IV) 29 In situ hybridization (IV) 29 Modeling of three dimensional structure (IV) 30
Source: Työväentutkimus Vuosikirja
Author: Nina Korsisaari
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: Helsingin Yliopisto
OpenAlex ID: W2156379270