Work

Two Cellobiohydrolase-Encoding Genes from Aspergillus niger Require d -Xylose and the Xylanolytic Transcriptional Activator XlnR for Their Expression

Year: 1999

Type: article

Abstract: Two cellobiohydrolase-encoding genes, cbhA and cbhB, have been isolated from the filamentous fungus Aspergillus niger. The deduced amino acid sequence shows that CbhB has a modular structure consisting of a fungus-type cellulose-binding domain (CBD) and a catalytic domain separated by a Pro/Ser/Thr-rich linker peptide. CbhA consists only of a catalytic domain and lacks a CBD and linker peptide. Both proteins are homologous to fungal cellobiohydrolases in family 7 of the glycosyl hydrolases. Northern blot analysis showed that the transcription of the cbhA and cbhB genes is induced by D-xylose but not by sophorose and, in addition, requires the xylanolytic transcriptional activator XlnR.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.65.10.4340-4345.1999

Authors: Marco M. C. Gielkens, Ester Dekkers, Jaap Visser, Leo H. de Graaff

Institution: Wageningen University & Research

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2156545952

FWCI: 1.533

Cites: 40

Cited by: 208

Related to: 10